TREATMENT OF ANTIBODIES TO REDUCE NONSPECIFIC-BINDING IN IMMUNOASSAYS USING THE AVIDIN-BIOTIN COMPLEX

被引:8
作者
BODMER, DM [1 ]
TIEFENAUER, LX [1 ]
机构
[1] PAUL SCHERRER INST,MED BIOANALYT PROJECT,CH-5232 VILLIGEN PSI,SWITZERLAND
来源
JOURNAL OF IMMUNOASSAY | 1990年 / 11卷 / 02期
关键词
Antibody; avidin; biotin; enzyme immunoassay; non-specific binding;
D O I
10.1080/01971529008053265
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three different methods to reduce non-specific binding of antibodies to avidin coated plates in an immunoassay are described and compared: The use of a blocking agent, antiserum adsorption on avidin-agarose and antiserum adsorption to avidin by a so called pre-incubation method. The pre-incubation method proved to be highly efficient, fast and simple. Further applications of this method to different immunoassay systems are proposed in the discussion section. © 1990, Marcel Dekker, Inc.
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页码:139 / 145
页数:7
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