HISTONE ACETYLTRANSFERASES AND HISTONE DEACETYLASES OF PHYSARUM-POLYCEPHALUM

被引：9

作者：

BROSCH, G ^{[1
]}

LOPEZRODAS, G ^{[1
]}

GOLDERER, G ^{[1
]}

LINDNER, H ^{[1
]}

GROBNER, P ^{[1
]}

LOIDL, P ^{[1
]}

机构：

[1] UNIV INNSBRUCK,SCH MED,DEPT MED CHEM & BIOCHEM,A-6020 INNSBRUCK,AUSTRIA

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1992年 / 16卷 / 11期

关键词：

D O I：

10.1016/S0309-1651(05)80036-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

DEAE-Sepharose chromatography of plasmodial extracts of the myxomycete Physarum polycephalum reveals the presence of multiple histone acetyltransferases and histone deacetylases. Five putative histone acetyltransferases and histone deacetylases. Five putative histone acetyltransferase forms with different substrate specificity can be discriminated: one enzyme which acetylates all core histones and four enzymes with a preference for each of H3, H2A, H2B or H4. Two histone deacetylases, HD1 and HD2, can be discriminated. They differ with respect to substrate specificity and pH-dependence. The substrate specificity of histone deacetylases is determined using HPLC-purified individual core histone species. The order of acetylated substrate preference is H2A>>H3≥H4> H2B for HD1, H3>H2A>H4 for HD2, respectively; HD2 is inactive with H2B as substrate. © 1992 Academic Press Ltd.

引用

页码：1103 / 1109

页数：7

共 17 条

[1] A NOVEL YEAST HISTONE DEACETYLASE - PARTIAL CHARACTERIZATION AND DEVELOPMENT OF AN ACTIVITY ASSAY [J].

ALONSO, WR ;

NELSON, DA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 866 (2-3) :161-169

[2]

Daniel J. W., 1964, METHOD CELL PHYSIOL, P9

[3] HISTONE ACETYLTRANSFERASE ACTIVITY DURING THE CELL-CYCLE [J].

GOLDERER, G ;

LOIDL, P ;

GROBNER, P .

FEBS LETTERS, 1987, 222 (02) :322-326

[4] A NEW HPLC ISOLATION PROCEDURE FOR CHICKEN AND GOOSE ERYTHROCYTE HISTONES [J].

HELLIGER, W ;

LINDNER, H ;

HAUPTLORENZ, S ;

PUSCHENDORF, B .

BIOCHEMICAL JOURNAL, 1988, 255 (01) :23-27

[5] LACK OF CORRELATION BETWEEN HISTONE-H4 ACETYLATION AND TRANSCRIPTION DURING THE PHYSARUM CELL-CYCLE [J].

LOIDL, P ;

LOIDL, A ;

PUSCHENDORF, B ;

GROBNER, P .

NATURE, 1983, 305 (5933) :446-448

[6]

LOIDL P, 1987, J BIOL CHEM, V262, P10195

[7] POSTSYNTHETIC ACETYLATION OF HISTONES DURING THE CELL-CYCLE - A GENERAL FUNCTION FOR THE DISPLACEMENT OF HISTONES DURING CHROMATIN REARRANGEMENTS [J].

LOIDL, P ;

GROBNER, P .

NUCLEIC ACIDS RESEARCH, 1987, 15 (20) :8351-8366

[8] TOWARDS AN UNDERSTANDING OF THE BIOLOGICAL FUNCTION OF HISTONE ACETYLATION [J].

LOIDL, P .

FEBS LETTERS, 1988, 227 (02) :91-95

[9]

LOPEZRODAS G, 1989, J BIOL CHEM, V264, P19028

[10] ENZYMES INVOLVED IN THE DYNAMIC EQUILIBRIUM OF CORE HISTONE ACETYLATION OF PHYSARUM-POLYCEPHALUM [J].

LOPEZRODAS, G ;

BROSCH, G ;

GOLDERER, G ;

LINDNER, H ;

GROBNER, P ;

LOIDL, P .

FEBS LETTERS, 1992, 296 (01) :82-86

← 1 2 →