SIMULTANEOUS MEASUREMENT OF CONTRACTION AND OXYGEN-CONSUMPTION IN CARDIAC MYOCYTES

A setup has been developed that simultaneously measures the mechanics and the energies of electrically induced contractions at physiological frequencies of isolated cardiac myocytes. The core of the setup is a self-manufactured stimulation chamber in which most of the myocytes are in suspension while some are attached to a plastic cover slip prepared from culture Petri dishes. The analysis of the contractile behavior of the attached myocytes is based on an image-processing system with digitized frames of a charge-coupled device camera. Thirty-six frames illuminated by a stroboscope are taken at increasing time intervals between stimulus and flash (snap), allowing one to resolve the contraction cycle with a very high time resolution (down to 1 ms). The number of pixels that differ between each of these frames and a "reference" frame of the cells in the relaxed state (slack cell length) are used to quantify the contractions. An oxgygen electrode in the chamber registers the drop of oxygen tension resulting from the consumption by the myocytes, which exhibit a strictly aerobic metabolism. The resulting data are also stored and analyzed in an IBM-AT-compatible computer.