THE USE OF HUMAN LUNG SLICES IN TOXICOLOGY

被引:62
作者
FISHER, RL [1 ]
SMITH, MS [1 ]
HASAL, SJ [1 ]
HASAL, KS [1 ]
GANDOLFI, AJ [1 ]
BRENDEL, K [1 ]
机构
[1] PROCTER & GAMBLE CO,IVORYDALE TECH CTR,CINCINNATI,OH 45217
来源
HUMAN & EXPERIMENTAL TOXICOLOGY | 1994年 / 13卷 / 07期
关键词
D O I
10.1177/096032719401300703
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
1 Successful use of agar-filled precision-cut rat lung slices in dynamic organ culture prompted the use of this technology with human lung. 2 The larger tissue mass of a human lung required that the trachea be cannulated with a balloon catheter and subsequently inflated with 4 liters of warm agar/medium mixture and then cooled before being precision-cut into 500 mu m thick slices. 3 To characterize the human lung slices, viability and the effects of acrolein and nitrofurantoin were assessed over a period of 24 h using protein synthesis and nonprotein sulfhydryl content. 4 Control human lung slices synthesized protein at a linear rate and maintained a stable nonprotein sulfhydryl content for 24 h. 5 Slices incubated with acrolein exhibited no significant decrease in protein synthesis or nonprotein sulfhydryl levels until 24 h. 6 Incubation with nitrofurantoin exhibited a definite time- and dose-dependent inhibition of protein synthesis, and depletion of the cellular thiol pool. 7 These results indicate that this human lung tissue slice system may be used as an in vitro model to identify and screen pneumotoxicants.
引用
收藏
页码:466 / 471
页数:6
相关论文
共 25 条
[1]   AN EVALUATION OF THE REDOX CYCLING POTENCIES OF PARAQUAT AND NITROFURANTOIN IN MICROSOMAL AND LUNG SLICE SYSTEMS [J].
ADAM, A ;
SMITH, LL ;
COHEN, GM .
BIOCHEMICAL PHARMACOLOGY, 1990, 40 (07) :1533-1539
[2]   A CRITICAL-REVIEW OF THE LITERATURE ON ACROLEIN TOXICITY [J].
BEAUCHAMP, RO ;
ANDJELKOVICH, DA ;
KLIGERMAN, AD ;
MORGAN, KT ;
HECK, HD .
CRC CRITICAL REVIEWS IN TOXICOLOGY, 1985, 14 (04) :309-380
[3]   CHRONIC NITROFURANTOIN REACTION ASSOCIATED WITH LYMPHOCYTE-T ALVEOLITIS [J].
BRUTINEL, WM ;
MARTIN, WJ .
CHEST, 1986, 89 (01) :150-152
[4]  
CHUDNOFSKY C R, 1989, Journal of Emergency Medicine, V7, P15, DOI 10.1016/0736-4679(89)90403-4
[5]   NITROFURANTOIN [J].
DARCY, PF .
DRUG INTELLIGENCE & CLINICAL PHARMACY, 1985, 19 (7-8) :540-547
[6]   VALPROIC ACID HEPATOTOXICITY IN HUMAN LIVER SLICES [J].
FISHER, R ;
NAU, H ;
GANDOLFI, AJ ;
PUTNAM, CW ;
BRENDEL, K .
DRUG AND CHEMICAL TOXICOLOGY, 1991, 14 (04) :375-394
[7]   COLDPRESERVATION AND CRYOPRESERVATION OF HUMAN LIVER AND KIDNEY SLICES [J].
FISHER, RL ;
HASAL, SJ ;
SANUIK, JT ;
SCOTT, KS ;
GANDOLFI, AJ ;
BRENDEL, K .
CRYOBIOLOGY, 1993, 30 (03) :250-261
[8]   ADVERSE REACTIONS TO NITROFURANTOIN - ANALYSIS OF 921 REPORTS [J].
HOLMBERG, L ;
BOMAN, G ;
BOTTIGER, LE ;
ERIKSSON, B ;
SPROSS, R ;
WESSLING, A .
AMERICAN JOURNAL OF MEDICINE, 1980, 69 (05) :733-738
[9]   A NEW INSTRUMENT FOR THE RAPID PREPARATION OF TISSUE-SLICES [J].
KRUMDIECK, CL ;
DOSSANTOS, JE ;
HO, KJ .
ANALYTICAL BIOCHEMISTRY, 1980, 104 (01) :118-123
[10]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265