A PCR-BASED METHOD TO AMPLIFY DNA WITH RANDOM PRIMERS - DETERMINING THE CHROMOSOMAL CONTENT OF PORCINE FLOW-KARYOTYPE PEAKS BY CHROMOSOME PAINTING

被引：25

作者：

MILAN, D

YERLE, M

SCHMITZ, A

CHAPUT, B

VAIMAN, M

FRELAT, G

GELLIN, J

机构：

[1] INRA,RADIOBIOL APPL LAB,F-78350 JOUY EN JOSAS,FRANCE

[2] CEA,CYTOMETRIE LAB,F-92260 FONTENAY ROSES,FRANCE

来源：

CYTOGENETICS AND CELL GENETICS | 1993年 / 62卷 / 2-3期

关键词：

D O I：

10.1159/000133457

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We present here a new PCR-based technique that allows the production of several micrograms of DNA from only 300 flow-sorted chromosomes. During the first two PCR cycles, the annealing temperature is decreased to 30-degrees-C, and numerous random loci are amplified under nonspecific conditions. As demonstrated here for pig chromosomes 1 and 18, the PCR products may be used to identify the chromosomal content of the flow-karyotype peaks of any species by fluorescence in situ hybridization.

引用

页码：139 / 141

页数：3

共 8 条

[1]

ARCHIBALD A, 1991, ANIM GENET S1, V22, P82

[2]

Grunwald D., 1989, FLOW CYTOMETRY ADV R, P131

[3] FLUORESCENCE INSITU HYBRIDIZATION WITH ALU AND L1 POLYMERASE CHAIN-REACTION PROBES FOR RAPID CHARACTERIZATION OF HUMAN-CHROMOSOMES IN HYBRID CELL-LINES [J].