DETERMINATION OF MORPHINE-3-GLUCURONIDE IN HUMAN URINE BY CAPILLARY ZONE ELECTROPHORESIS AND MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

被引：46

作者：

WERNLY, P

THORMANN, W

BOURQUIN, D

BRENNEISEN, R

机构：

[1] UNIV BERN,DEPT CLIN PHARMACOL,MURTENSTRASSE,CH-3010 BERN,SWITZERLAND

[2] UNIV BERN,INST PHARM,CH-3012 BERN,SWITZERLAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 616卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80399-O

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Attempts to determine morphine-3-glucuronide (MO3G) by high-performance capillary electrophoresis and micellar electrokinetic capillary chromatography are reported. Using direct injection of urine, it was possible to achieve a limit of detection of about 20 mug/ml, which is poor compared with high-performance liquid chromatography and immunoassays. However, employing sample extraction with C-8 cartridges, the presence of MO3G in urines that tested positive for opioids using a commercial enzyme-multiplied immunoassay technique could be successfully confirmed. The limit of detection with unambiguous identification of MO3G via spectral analysis was about 1 mug/ml.

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页码：305 / 310

页数：6

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