TRANSFER OF PSEUDOMONAS-PLANTARII AND PSEUDOMONAS-GLUMAE TO BURKHOLDERIA AS BURKHOLDERIA SPP AND DESCRIPTION OF BURKHOLDERIA-VANDII SP-NOV

Plant-associated bacteria were characterized and are discussed in relation to authentic members of the genus Pseudomonas sensu stricto. Bacteria belonging to Pseudomonas rRNA group I! are separated clearly from members of the genus Pseudomonas sensu stricto (Pseudomonas florescens rRNA group) on the basis of plant association characteristics, chemotaxonomic characteristics, DNA-DNA hybridization data, rRNA-DNA hybridization data, and the sequences of 5S and 16S rRNAs. The transfer of Pseudomonas cepacia, Pseudomonas mallei, Pseudomonas pseudomallei, Pseudomonas caryophylli, Pseudomonas gladioli, Pseudomonas pickettii, and Pseudomonas solanacearum to the new genus Burkholderia is supported; we also propose that Pseudomonas plantarii and Pseudomonas glumae should be transferred to the genus Burkholderia. Isolate VA-1316(T) (T = type strain) was distinguished from Burkholderia species on the basis of physiological characteristics and DNA-DNA hybridization data. A new species, Burkholderia vandii sp. nov. is proposed for this organism; the type strain of B. vandii is VA-1316 (= JCM 7957).