Lipid peroxidation product, 4-hydroxynonenal and its conjugate with GSH are excellent substrates of bovine lens aldose reductase

被引:159
作者
Srivastava, S [1 ]
Chandra, A [1 ]
Bhatnagar, A [1 ]
Srivastava, SK [1 ]
Ansari, NH [1 ]
机构
[1] UNIV TEXAS,MED BRANCH,DEPT HUMAN BIOL CHEM & GENET,GALVESTON,TX 77555
关键词
D O I
10.1006/bbrc.1995.2835
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There is increasing evidence that lipid aldehydes generated endogenously during the process of degradation of lipid peroxides, are causally involved in the pathophysiology effects associated with oxidative stress. We report here that 4-hydroxynonenal (HNE), which is one of the most abundant and toxic lipid aldehyde can be efficiently detoxified by the aldo-keto reductase, aldose reductase, purified from bovine lens. The enzyme displays a K-m of approximate to 9 mu M for HNE and 34 mu M for the glutathione adduct of HNE (HNE-GS) assigning HNE and HNE-GS to be the best natural substrates of aldose reductase known so far and exposing a new efficient detoxification route of HNE. (C) 1995 Academic Press, Inc.
引用
收藏
页码:741 / 746
页数:6
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