SERUM-FREE AND SERUM-CONTAINING MEDIA FOR GROWTH OF SUSPENDED BHK AGGREGATES IN STIRRED VESSELS

被引:11
作者
MOREIRA, JL
ALVES, PM
FELICIANO, AS
AUNINS, JG
CARRONDO, MJT
机构
[1] INST TECNOL QUIM & BIOL, INST BIOL EXPTL & TECNOL, P-2780 OEIRAS, PORTUGAL
[2] MERCK & CO INC, W POINT, PA USA
[3] UNL, FCT, ENGN BIOQUIM LAB, MONTE DE CAPARICA, PORTUGAL
关键词
AGITATION; BHK CELL GROWTH; NATURAL AGGREGATES; SERUM; SERUM-FREE MEDIUM; STIRRED VESSELS;
D O I
10.1016/0141-0229(94)00071-X
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Baby hamster kidney (BHK) cells were grown in stirred spinner flasks at 45 rpm, using both serum-containing and serum-free media. In both studies natural aggregates were formed. Aggregate formation is independent of cell growth or medium composition, but aggregate final properties are dependent on cell growth. Serum concentrations were varied between 0 and 20% without the addition of any serum substitutes. Aggregate characteristics (diameter, fraction of cells in aggregates, and compactness) remained constant above 5% serum, clearly indicating that there is no direct dependence of aggregate properties on serum above that concentration. Cell growth decreased with serum concentration below 5%. A serum-free medium with similar basal medium and supplemented with several additives was tested. Cell growth, cell viability, and aggregate characteristics remained unchanged when serum-free medium was compared with serum-containing medium with more than 5% serum at the same agitation rate. Using the serum-free medium, agitation rare was varied between 25 and 60 rpm. No differences in cell growth or viability were observed, but aggregate diameter was largely reduced with the increase in agitation rate (from 200 to 90 mu m); this clearly indicates that the control of the size of aggregates by vessel hydrodynamics previously reported for serum-containing medium is independent of the medium composition including absence or presence of serum, for the two tested media.
引用
收藏
页码:437 / 444
页数:8
相关论文
共 28 条
[1]   SERUM-FREE CELL-CULTURE - A UNIFYING APPROACH [J].
BARNES, D ;
SATO, G .
CELL, 1980, 22 (03) :649-655
[2]  
BORASTON R, 1992, ANIMAL CELL TECHNOLOGY : DEVELOPMENTS, PROCESSES AND PRODUCTS, P424
[3]   NUTRITIONAL ASPECTS OF THE GROWTH OF ANIMAL-CELLS IN CULTURE [J].
BUTLER, M ;
JENKINS, H .
JOURNAL OF BIOTECHNOLOGY, 1989, 12 (02) :97-110
[4]   GROWTH AND DEATH IN OVERAGITATED MICROCARRIER CELL-CULTURES [J].
CROUGHAN, MS ;
WANG, DIC .
BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (06) :731-744
[5]   HYDRODYNAMIC EFFECTS ON ANIMAL-CELLS GROWN IN MICROCARRIER CULTURES [J].
CROUGHAN, MS ;
HAMEL, JF ;
WANG, DIC .
BIOTECHNOLOGY AND BIOENGINEERING, 1987, 29 (01) :130-141
[6]   TRANSIENT KINETICS OF HYBRIDOMA GROWTH AND MONOCLONAL-ANTIBODY PRODUCTION IN SERUM-LIMITED CULTURES [J].
DALILI, M ;
OLLIS, DF .
BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (08) :984-990
[7]   MATHEMATICAL DESCRIPTIONS OF HYBRIDOMA CULTURE KINETICS .2. THE RELATIONSHIP BETWEEN THIOL CHEMISTRY AND THE DEGRADATION OF SERUM ACTIVITY [J].
GLACKEN, MW ;
ADEMA, E ;
SINSKEY, AJ .
BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (04) :440-450
[8]  
GOETGHEBEUR S, 1991, APPL MICROBIOL BIOT, V34, P735
[9]  
Jager V, 1988, Cytotechnology, V1, P319, DOI 10.1007/BF00365077
[10]  
JORDAN M, 1992, ANIMAL CELL TECHNOLOGY : DEVELOPMENTS, PROCESSES AND PRODUCTS, P418