ASSOCIATION OF A CYTOPLASMIC DYNEIN-LIKE PROTEIN RECOGNIZABLE BY ANTI-MAP 1C WITH THE MAMMALIAN MITOTIC SPINDLE

被引：8

作者：

YOSHIDA, T

KATSUTA, K

TAKANARI, H

IZUTSU, K

机构：

[1] Department of Pathology, Mie University School of Medicine, Tsu

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1990年 / 14卷 / 03期

关键词：

D O I：

10.1016/S0309-1651(05)80001-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A rabbit antibody to bovine brain MAP 1C was prepared. The antibody stained the mitotic spindle of PtK2 cells by immunofluorescence. On immunoblots of PtK2 cell extract the antibody reacted with polypeptides of molecular weights >350 and 80 KD that resemble the subunit proteins of bovine brain MAP 1C. An additional 135 KD polypeptide in the extract was also stained. These results indicate that a cytoplasmic dynein recognizable by the anti-MAP 1C antibody is localized in the mitotic spindle. © 1990 Academic Press Ltd.

引用

页码：189 / 198

页数：10

共 23 条

[1] PHYSIOLOGICAL AND ULTRASTRUCTURAL ANALYSIS OF ELONGATING MITOTIC SPINDLES REACTIVATED INVITRO [J].