DETECTION OF DIPHTHERIA-TOXIN IN CULTURE SUPERNATES OF CORYNEBACTERIUM-DIPHTHERIAE AND C-ULCERANS BY IMMUNOASSAY WITH MONOCLONAL-ANTIBODY

被引：7

作者：

HALLAS, G ^{[1
]}

HARRISON, TG ^{[1
]}

SAMUEL, D ^{[1
]}

COLMAN, G ^{[1
]}

机构：

[1] CENT PUBL HLTH LAB,DIV HOSP INFECT,LONDON NW9 5HT,ENGLAND

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 1990年 / 32卷 / 04期

关键词：

D O I：

10.1099/00222615-32-4-247

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Monoclonal antibody (MAb) to diphtheria toxin was produced in mouse hybridomas, and shown by ELISA to be of sub-class IgG1. Hybridomas were inoculated into mice to produce ascitic fluid from wich MAb was purified by caprylic acid. The MAb was shown by immunoblotting to be directed against the A fragment of the toxin and also against the intact toxin molecule. After conjugation with fluorescein isothiocyanate, it was used in an immunoassay to detect toxin in culture supernates of Corynebacterium diphtheriae and C. ulcerans. The assay correlated well with the Elek test and with virulence in guinea-pigs; but it gave occasional false positive results, probably by binding of MAb to defective toxin.

引用

页码：247 / 253

页数：7

共 24 条

[1]

BARKSDALE L, 1970, Bacteriological Reviews, V34, P378

[2] AN OUTBREAK OF DIPHTHERIA AMONG SWEDISH ALCOHOLICS [J].

BJORKHOLM, B ;

OLLING, S ;

LARSSON, P ;

HAGBERG, L .

INFECTION, 1987, 15 (05) :354-358

[3]

DAVIES JR, 1974, PUBLIC HLTH LABORATO, V5, P1

[4] THE USE OF SDS-POLYACRYLAMIDE GEL-ELECTROPHORESIS IN EPIDEMIOLOGICAL-STUDIES OF CORYNEBACTERIUM-DIPHTHERIAE [J].

HALLAS, G .

EPIDEMIOLOGY AND INFECTION, 1988, 100 (01) :83-90

[5]

HAYAKAWA S, 1983, J BIOL CHEM, V258, P4311

[6]

Ionescu M, 1986, Arch Roum Pathol Exp Microbiol, V45, P5

[7] DIPHTHERIA OUTBREAKS IN IMMUNIZED POPULATIONS [J].

KARZON, DT ;

EDWARDS, KM .

NEW ENGLAND JOURNAL OF MEDICINE, 1988, 318 (01) :41-43

[8]

KRECH T, 1987, ZBL BAKT-INT J MED M, V265, P124

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] RAPID AND SENSITIVE COLORIMETRIC METHOD FOR VISUALIZING BIOTIN-LABELED DNA PROBES HYBRIDIZED TO DNA OR RNA IMMOBILIZED ON NITROCELLULOSE - BIO-BLOTS [J].

LEARY, JJ ;

BRIGATI, DJ ;

WARD, DC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13) :4045-4049

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