PERTURBATIONS IN DNA-STRUCTURE UPON INTERACTION WITH PORPHYRINS REVEALED BY CHEMICAL PROBES, DNA FOOTPRINTING AND MOLECULAR MODELING

被引：31

作者：

FORD, KG ^{[1
]}

NEIDLE, S ^{[1
]}

机构：

[1] INST CANC RES,CRC BIOMOLEC STRUCT UNIT,SUTTON SM2 5NG,SURREY,ENGLAND

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1995年 / 3卷 / 06期

关键词：

D O I：

10.1016/0968-0896(95)00052-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interactions of several porphyrins with a 74 base-pair DNA sequence have been examined by footprinting and chemical protection methods. Tetra-(4-N-methyl-(pyridyl)) porphyrin (TMPy), two of its metal complexes and tetra-(4-trimethylanilinium) porphyrin (TMAP) bind to closely similar AT-rich sequences. The three TMPy ligands produce modest changes in DNA structure and base accessibility on binding, in contrast to the large-scale conformational changes observed with TMAP. Molecular modelling studies have been performed on TMPy and TMAP bound in the AT-rich minor groove of an oligonucleotide. These have shown that significant structural change is needed to accommodate the bulky trimethyl substituent groups of TMAP, in contrast to the facile minor groove fit of TM4y.

引用

页码：671 / 677

页数：7

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