SPECIFIC ENZYMATIC MICROASSAYS OF ALPHA-AMYLASE AND BETA-AMYLASE IN CEREALS

被引:38
作者
SIROU, Y [1 ]
LECOMMANDEUR, D [1 ]
LAURIERE, C [1 ]
机构
[1] CNRS,PHYSIOL ORGANES VEGETAUX,4 ROUTE GARDES,F-92190 MEUDON,FRANCE
关键词
D O I
10.1021/jf00091a037
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Two colorimetric assays for specific determination in cereals of α- and β-amylase, respectively, were adapted for use in microtiter plates. The two micromethods allow easy and reliable determinations of -α and β-amylase in a large number of samples. Application is provided with Km determination of barley, maize, sorghum, and rice α- and β-amylases, respectively. © 1990, American Chemical Society. All rights reserved.
引用
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页码:171 / 174
页数:4
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