CRYSTALLIZATION AND PRELIMINARY CHARACTERIZATION OF MITOGILLIN, A RIBOSOMAL RIBONUCLEASE FROM ASPERGILLUS-RESTRICTUS

被引：11

作者：

MARTINEZ, SE ^{[1
]}

SMITH, JL ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT BIOL SCI,W LAFAYETTE,IN 47907

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 218卷 / 03期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0022-2836(91)90692-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitogillin is a ribonuclease secreted by the fungus Aspergillus restrictus. The substrate for mitogillin is a short, universally conserved, sequence in ribosomal RNA. Cleavage of this sequence inactivates protein synthesis. Mitogillin was crystallized by a two-chamber vapor/ liquid diffusion method using ethanol as the precipitant. This method has wider potential in the use of volatile organic solvents as precipitants. Crystals of mitogillin diffract X-rays to lattice d-spacings of at least 1.6 Å, and belong to the monoclinic space group P21. with a = 50.4 A ̊, b = 82.4 A ̊, c = 38.2 A ̊and β = 99.8°. © 1991.

引用

页码：489 / 492

页数：4

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