DNA POLYMERASE-ALPHA-PRIMASE COMPLEX OF PHYSARUM-POLYCEPHALUM

被引：7

作者：

ACHHAMMER, G ^{[1
]}

ANGERER, B ^{[1
]}

WINDISCH, C ^{[1
]}

UHL, A ^{[1
]}

HOLLER, E ^{[1
]}

机构：

[1] INST BIOPHYS & PHYS BIOCHEM,UNIV STR 31,W-8400 REGENSBURG,GERMANY

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1992年 / 16卷 / 11期

关键词：

D O I：

10.1016/S0309-1651(05)80028-1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

DNA polymerase α and DNA polymerase α - primase complex of Physarum polycephalum were purified by rapid methods, and antibodies were raised against the complex. In crude extracts, immune-reactive polypeptides of 220 kDa, 180 kDa, 150 kDa, 140 kDa, 110 kDa, 86 kDa, 57 kDa and 52 kDa were identified. The structural relationships between the 220 kDa, 110 kDa and 140 kDa (the most abundant form) was investigated by peptide mapping. The 140 kDa form was active DNA polymerase α. The 57 kDa and the 52 kDa polypeptides were identified as primase subunits by auto-catalytic labelling. In amoebae, the immune-reactive 140 kDa polypeptide was replaced by a 135 kDa active DNA polymerase α. © 1992 Academic Press Ltd.

引用

页码：1047 / 1053

页数：7

共 11 条

[1]

CLEVELAND DW, 1977, J BIOL CHEM, V252, P1102

[2]

Daniel J.W., 1964, METHODS CELL BIOL, V1, P9, DOI [10.1016/S0091-679X(08)62084-1, DOI 10.1016/50091-679X(08)62084-1]

[3]

DEE J, 1989, J GEN MICROBIOL, V135, P1567

[4]

FIOANI M, 1989, J BIOL CHEM, V264, P2189

[5] A DNA-POLYMERASE WITH UNUSUAL PROPERTIES FROM THE SLIME-MOLD PHYSARUM-POLYCEPHALUM [J].

HOLLER, E ;

FISCHER, H ;

WEBER, C ;

STOPPER, H ;

STEGER, H ;

SIMEK, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 163 (02) :397-405

[6]

HOLLER E, 1992, IN PRESS EUR J BIOCH

[7] ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].

TOWBIN, H ;

STAEHELIN, T ;

GORDON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354

[8]

Vaitukaitis J L, 1981, Methods Enzymol, V73, P46

[9] EUKARYOTIC DNA-POLYMERASES [J].

WANG, TSF .

ANNUAL REVIEW OF BIOCHEMISTRY, 1991, 60 :513-552

[10] PURIFICATION AND CHARACTERIZATION OF DNA POLYMERASE-ALPHA FROM PLASMODIA OF PHYSARUM-POLYCEPHALUM [J].

WEBER, C ;

FISCHER, H ;

HOLLER, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 176 (01) :199-206

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