THE DEWAR VALENCE ISOMER OF THE (6-4) PHOTOADDUCT OF THYMIDYLYL-(3'-5')-THYMIDINE MONOPHOSPHATE - FORMATION, ALKALINE LABILITY AND CONFORMATIONAL PROPERTIES

被引：33

作者：

KAN, LS

VOITURIEZ, L

CADET, J

机构：

[1] CEN,SESM,DRFMC,LES ACIDES NUCL LAB,85X,F-38041 GRENOBLE,FRANCE

[2] JOHNS HOPKINS UNIV,DEPT BIOCHEM,BALTIMORE,MD 21205

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1992年 / 12卷 / 04期

关键词：

TPT PHOTOPRODUCTS; PYRIMIDINE(6-4)PYRIMIDONE PHOTOADDUCTS; DEWAR ISOMER; PHOTOISOMERIZATION;

D O I：

10.1016/1011-1344(92)85040-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The formation of the Dewar valence isomer of the pyrimidine(6-4)pyrimidone photoadduct of thymidylyl-(3'-5')-thymidine monophosphate (TpT) was investigated under different irradiation conditions. This photoproduct was generated on exposure of TpT to far-UV radiation. However, no detectable amount of the Dewar isomer or its precursor (pyrimidine(6-4)pyrimidone photoadduct) was observed following acetone photosensitization of TpT. The Dewar valence isomer was much more unstable than the pyrimidine(6-4)pyrimidone photoproduct when treated with hot piperidine. A detailed conformational analysis of the TpT Dewar isomer photoproduct is reported as inferred from extensive one- and two-dimensional 300 and 620 MHz proton nuclear magnetic resonance (H-1 NMR) measurements and molecular mechanics calculations.

引用

页码：339 / 357

页数：19

共 51 条

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