SEPARATION AND QUANTITATION OF THE AMINO-ACID NEUROTRANSMITTERS IN RAT-BRAIN BY CAPILLARY ELECTROPHORESIS

被引：29

作者：

WEBER, PL

OSHEA, TJ

LUNTE, SM

机构：

[1] BRIAR CLIFF COLL,DEPT CHEM,SIOUX CITY,IA 51104

[2] UNIV KANSAS,CTR BIOANALYT RES,LAWRENCE,KS 66047

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1994年 / 12卷 / 03期

基金：

美国国家科学基金会;

关键词：

CAPILLARY ELECTROPHORESIS; AMINO ACID NEUROTRANSMITTERS; RAT BRAIN; NAPHTHALENE-2,3-DICARBOXYALDEHYDE;

D O I：

10.1016/0731-7085(94)90006-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary electrophoresis (CE) has been used to separate the CBI derivatives of the neurotransmitters gamma-aminobutyric acid, glycine, glutamate, aspartate, norepinephrine and dopamine from 18 other amino acids present in the rat brain. The procedure, which requires an injection volume of <10 nl, gave detection limits of 24-40 fmol using UV detection at 420 nm. Efficiencies for the derivatized amino acids varied from 344,000 to 444,000 theoretical plates. The method was applied successfully to the quantitation of the amino acid neurotransmitters and several other amino acids in a rat brain homogenate.

引用

收藏

页码：319 / 324

页数：6

相关论文

共 18 条

[1] ORTHO-PHTHALALDEHYDE DERIVATIVES OF AMINES FOR HIGH-SPEED LIQUID-CHROMATOGRAPHY ELECTROCHEMISTRY [J].

ALLISON, LA ;

MAYER, GS ;

SHOUP, RE .

ANALYTICAL CHEMISTRY, 1984, 56 (07) :1089-1096

[2] APPLICATION OF SAMPLE STACKING TO GRAVITY INJECTION IN CAPILLARY ELECTROPHORESIS [J].

BURGI, DS ;

CHIEN, RL .

JOURNAL OF MICROCOLUMN SEPARATIONS, 1991, 3 (03) :199-202

[3]

COOPER JR, 1986, BIOCH BASIS NEUROPHA, P125

[4] NAPHTHALENE-2,3-DICARBOXALDEHYDE CYANIDE ION - A RATIONALLY DESIGNED FLUOROGENIC REAGENT FOR PRIMARY AMINES [J].

DEMONTIGNY, P ;

STOBAUGH, JF ;

GIVENS, RS ;

CARLSON, RG ;

SRINIVASACHAR, K ;

STERNSON, LA ;

HIGUCHI, T .

ANALYTICAL CHEMISTRY, 1987, 59 (08) :1096-1101

[5] ELECTROCHEMICAL ACTIVITY OF ORTHO-PHTHALALDEHYDE MERCAPTOETHANOL DERIVATIVES OF AMINO-ACIDS - APPLICATION TO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMINO-ACIDS IN PLASMA AND OTHER BIOLOGICAL-MATERIALS [J].

JOSEPH, MH ;

DAVIES, P .

JOURNAL OF CHROMATOGRAPHY, 1983, 277 (OCT) :125-136

[6] O-PHTHALALDEHYDE AND THE FLUOROGENIC DETECTION OF PEPTIDES [J].

JOYS, TM ;

KIM, H .

ANALYTICAL BIOCHEMISTRY, 1979, 94 (02) :371-377

[7] MICROCOLUMN SEPARATIONS AND THE ANALYSIS OF SINGLE CELLS [J].

KENNEDY, RT ;

OATES, MD ;

COOPER, BR ;

NICKERSON, B ;

JORGENSON, JW .

SCIENCE, 1989, 246 (4926) :57-63

[8]

Lunte S.M., 1990, CURR SEP, V10, P19

[9] DETERMINATION OF DESMOSINE, ISODESMOSINE, AND OTHER AMINO-ACIDS BY LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION FOLLOWING PRECOLUMN DERIVATIZATION WITH NAPHTHALENEDIALDEHYDE CYANIDE [J].

LUNTE, SM ;

MOHABBAT, T ;

WONG, OS ;

KUWANA, T .

ANALYTICAL BIOCHEMISTRY, 1989, 178 (01) :202-207

[10] N-SUBSTITUTED 1-CYANOBENZ[F]ISOINDOLE - EVALUATION OF FLUORESCENCE EFFICIENCIES OF A NEW FLUOROGENIC LABEL FOR PRIMARY AMINES AND AMINO-ACIDS [J].

MATUSZEWSKI, BK ;

GIVENS, RS ;

SRINIVASACHAR, K ;

CARLSON, RG ;

HIGUCHI, T .

ANALYTICAL CHEMISTRY, 1987, 59 (08) :1102-1105