SIMULTANEOUS DETERMINATION OF VITAMIN-D-3, VITAMIN-E AND VITAMIN-K1, AND RETINYL PALMITATE IN CATTLE PLASMA BY LIQUID-CHROMATOGRAPHY WITH A NARROW-BORE COLUMN

被引:13
作者
GOMIS, DB
ARIAS, VJE
ALVAREZ, LEF
ALVAREZ, MDG
机构
[1] Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo
来源
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 660卷 / 01期
关键词
D O I
10.1016/0378-4347(94)00279-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A reversed-phase high performance liquid chromatographic method is described for the simultaneous determination of vitamins D-3, E and K-1 and retinyl palmitate in plasma. Narrow-bore columns are recommended because this alternative provides a good separation efficiency, plus greater economy and sensitivity. Detection limits for individual vitamins range from 0.42 to 2.8 ng. All vitamins were separated in less than 9 min. Recovery studies showed good results for all solutes (88.8-100.3%) and the intra-day coefficients of variations ranged from 1.0 to 4.5%. This method permits the simple determination of fat-soluble vitamins using 1 ml of cattle heparinized plasma.
引用
收藏
页码:49 / 55
页数:7
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