DENATURING GRADIENT GEL-ELECTROPHORESIS IDENTIFIES GENOMIC DNA POLYMORPHISM WITH HIGH-FREQUENCY IN MAIZE

被引：16

作者：

RIEDEL, GE

SWANBERG, SL

KURANDA, KD

MARQUETTE, K

LAPAN, P

BLEDSOE, P

KENNEDY, A

LIN, BY

机构：

[1] Genetics Institute, Inc., Cambridge, 02140, MA

来源：

THEORETICAL AND APPLIED GENETICS | 1990年 / 80卷 / 01期

关键词：

B-A translocation; Denaturing gradient gel electrophoresis; DNA polymorphism; Restriction fragment length polymorphism; Zea mays;

D O I：

10.1007/BF00224008

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We have used denaturing gradient gel electrophoresis (DGGE) to identify genomic DNA polymorphism in maize (Zea mays L.). DGGE probes detect polymorphism in maize at a frequency comparable to the incidence of restriction fragment length polymorphism (RFLP). Probes identifying polymorphism were mapped to maize chromosome arms by utilizing DGGE and maize lines carrying B-A chromosomal translocations. The methods for library construction, probe screening, and genome analysis, described here for maize, can also be applied to the genomic analysis of other organisms. © 1990 Springer-Verlag.

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页码：1 / 10

页数：10

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