IMPORT-DEFECTIVE COLICIN-B DERIVATIVES MUTATED IN THE TONB BOX

被引:60
作者
MENDE, J [1 ]
BRAUN, V [1 ]
机构
[1] UNIV TUBINGEN,W-7400 TUBINGEN 1,GERMANY
关键词
D O I
10.1111/j.1365-2958.1990.tb02063.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The pore‐forming colicin B is taken up into Escherichia coli by a receptor and TonB‐dependent process. The receptor and colicin B both contain a similar amino acid sequence, close to the N‐terminal end, termed the TonB box. Point mutations were introduced into the TonB‐box region of the colicin B structural gene cba resulting in colicin B derivatives which were partially or totally Inactive against E. coli cells. All derivatives still bound to the receptor. An inactive derivative killed cells when translocated across the outer membrane by osmotic shock treatment, and formed pores in planar lipid bilayer membranes identical to the wild‐type colicin. Some of the mutations were partially suppressed by mutations in the tonB structural gene. It was concluded that the TonB‐box mutations define a region that is involved in the uptake of colicin B across the outer membrane. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
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页码:1523 / 1533
页数:11
相关论文
共 38 条
[1]  
BERNSTEIN A, 1972, J BACTERIOL, V122, P74
[2]  
BIRNBOIM HC, 1983, METHOD ENZYMOL, V100, P243
[3]   PENETRATION OF COLICIN-M INTO CELLS OF ESCHERICHIA-COLI [J].
BRAUN, V ;
FRENZ, J ;
HANTKE, K ;
SCHALLER, K .
JOURNAL OF BACTERIOLOGY, 1980, 142 (01) :162-168
[5]   GENETICS OF RESISTANCE TO COLICINS IN ESCHERICHIA-COLI K-12 - CROSS-RESISTANCE AMONG COLICINS OF GROUP-A [J].
DAVIES, JK ;
REEVES, P .
JOURNAL OF BACTERIOLOGY, 1975, 123 (01) :102-117
[6]  
DAVIES JK, 1975, J BACTERIOL, V123, P96, DOI 10.1128/JB.123.1.96-101.1975
[7]   GENETICS AND PHYSIOLOGY OF COLICIN-TOLERANT MUTANTS OF ESCHERICHIA COLI [J].
DEZWAIG, RN ;
LURIA, SE .
JOURNAL OF BACTERIOLOGY, 1967, 94 (04) :1112-+
[8]   PHOSPHOROTHIOATES IN MOLECULAR-BIOLOGY [J].
ECKSTEIN, F ;
GISH, G .
TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (03) :97-100
[9]   IMPORT OF BIO-POLYMERS INTO ESCHERICHIA-COLI - NUCLEOTIDE-SEQUENCES OF THE EXBB AND EXBD GENES ARE HOMOLOGOUS TO THOSE OF THE TOLQ AND TOLR GENES, RESPECTIVELY [J].
EICKHELMERICH, K ;
BRAUN, V .
JOURNAL OF BACTERIOLOGY, 1989, 171 (09) :5117-5126
[10]   INVOLVEMENT OF EXBB AND TONB IN TRANSPORT ACROSS THE OUTER-MEMBRANE OF ESCHERICHIA-COLI - PHENOTYPIC COMPLEMENTATION OF EXB MUTANTS BY OVEREXPRESSED TONB AND PHYSICAL STABILIZATION OF TONB BY EXBB [J].
FISCHER, E ;
GUNTER, K ;
BRAUN, V .
JOURNAL OF BACTERIOLOGY, 1989, 171 (09) :5127-5134