A RECOGNITION MODEL OF TRANSFER-RNA SER BY SERYL-TRANSFER RNA-SYNTHETASE IN ESCHERICHIA-COLI

被引：19

作者：

ASAHARA, H

HIMENO, H

SHIMIZU, M

机构：

来源：

CHEMISTRY LETTERS | 1991年 / 03期

关键词：

D O I：

10.1246/cl.1991.363

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

E. coli seryl-tRNA synthetase (SerRS) is a dimeric enzyme, having a subunit composed of two domains, i.e., a globular domain that includes a putative active site, and a long antiparallel alpha-helical coiled coil domain. The recognition sites on tRNA(Ser) by SerRS were searched using the T7 transcription system, and a new model of the recognition mechanism was proposed, consisting of a long coil shaped domain on one subunit which interacts with the long extra arm of tRNA(Ser), and a globular domain of the other subunit which interacts with the 3' end of tRNA(Ser).

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页码：363 / 366

页数：4

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