NEW REAGENTS FOR THE INTRODUCTION OF THE THIOMETHYL GROUP AT SULFHYDRYL RESIDUES OF PROTEINS WITH CONCOMITANT SPECTROPHOTOMETRIC TITRATION OF THE SULFHYDRYLS - METHYL 3-NITRO-2-PYRIDYL DISULFIDE AND METHYL 2-PYRIDYL DISULFIDE

被引：22

作者：

KIMURA, T ^{[1
]}

MATSUEDA, R ^{[1
]}

NAKAGAWA, Y ^{[1
]}

KAISER, ET ^{[1
]}

机构：

[1] UNIV CHICAGO,DEPT CHEM,SEARLE CHEM LAB,CHICAGO,IL 60637

来源：

ANALYTICAL BIOCHEMISTRY | 1982年 / 122卷 / 02期

关键词：

D O I：

10.1016/0003-2697(82)90281-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：274 / 282

页数：9

共 23 条

[1]

Arnon R., 1970, METHOD ENZYMOL, V19, P226, DOI DOI 10.1016/0076-6879(70)19017-3

[2] DETERMINATION OF CONCENTRATION OF HYDROLYTIC ENZYME SOLUTIONS - ALPHA-CHYMOTRYPSIN TRYPSIN PAPAIN ELASTASE SUBTILISIN AND ACETYLCHOLINESTERASE [J].

BENDER, ML ;

BEGUECAN.ML ;

BLAKELEY, RL ;

BRUBACHER, LJ ;

FEDER, J ;

GUNTER, CR ;

KEZDY, FJ ;

KILLHEFFER, JV ;

MARSHALL, TH ;

MILLER, CG ;

ROESKE, RW ;

STOOPS, JK .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1966, 88 (24) :5890-+

[3] PURIFICATION OF PAPAIN BY AFFINITY CHROMATOGRAPHY [J].

BLUMBERG, S ;

SCHECHTER, I ;

BERGER, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1970, 15 (01) :97-+

[4] SPECTROPHOTOMETRIC STUDY OF THE REACTION OF PROTEIN SULFHYDRYL GROUPS WITH ORGANIC MERCURIALS [J].

BOYER, PD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1954, 76 (17) :4331-4337

[5] REACTIONS OF PAPAIN AND OF LOW-MOLECULAR-WEIGHT THIOLS WITH SOME AROMATIC DISULFIDES - 2,2'-DIPYRIDYL DISULFIDE AS A CONVENIENT ACTIVE-SITE TITRANT FOR PAPAIN EVEN IN PRESENCE OF OTHER THIOLS [J].

BROCKLEHURST, K ;

LITTLE, G .

BIOCHEMICAL JOURNAL, 1973, 133 (01) :67-80

[6]

Curd F. R. N., 1967, METHOD ENZYMOL, V1, P532

[7] CYANYLATION OF SULFHYDRYL GROUPS BY 2-NITRO-5-THIOCYANOBENZOIC ACID - HIGH-YIELD MODIFICATION AND CLEAVAGE OF PEPTIDES AT CYSTEINE RESIDUES [J].

DEGANI, Y ;

PATCHORNIK, A .

BIOCHEMISTRY, 1974, 13 (01) :1-11

[8] TISSUE SULFHYDRYL GROUPS [J].

ELLMAN, GL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) :70-77

[9]

FURLANETTO RW, 1970, J AM CHEM SOC, V92, P6980

[10]

GLAZER AN, 1961, J BIOL CHEM, V236, P2948

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