HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR AMILORIDE IN PLASMA AND URINE

被引：14

作者：

XU, DK ^{[1
]}

ZHOU, JH ^{[1
]}

YUAN, YS ^{[1
]}

LIU, XQ ^{[1
]}

HUANG, SK ^{[1
]}

机构：

[1] CHINA PHARMACEUT UNIV,NANJING 210009,PEOPLES R CHINA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 567卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80151-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and simplified high-performance liquid chromatographic procedure has been developed for quantification of amiloride in rabbit plasma, as well as human plasma and urine. Following protein precipitation with perchloric acid, the supernatant was directly injected into a C18 Nucleosil column. The mobile phase consisted of methanol-water (45:55) containing 0.1 M perchloric acid, and the compound was quantitated using a fluorescence detector at excitation and emission wavelengths of 286 and 418 nm, respectively. The average recovery was 97.6%. The calibration curve was linear over the range 2.0-20.0 ng/ml. The limit of detection was 0.5 ng/ml.

引用

页码：451 / 458

页数：8

共 8 条

[1]

BAER JE, 1967, J PHARMACOL EXP THER, V157, P472

[2] SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMILORIDE IN HUMAN-PLASMA BY DIRECT INJECTION WITH PRE-COLUMN ENRICHMENT [J].