A MOLECULAR FLUORESCENCE SPECTROMETER THAT CORRECTS FOR THE EFFECTS OF QUENCHING

被引：4

作者：

BOWMAN, LE ^{[1
]}

CROUCH, SR ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,DEPT CHEM,E LANSING,MI 48824

来源：

APPLIED SPECTROSCOPY | 1992年 / 46卷 / 06期

关键词：

MOLECULAR FLUORESCENCE SPECTROSCOPY; SPECTROSCOPIC TECHNIQUES; INSTRUMENTATION;

D O I：

10.1366/0003702924124330

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A fluorescence spectrometer has been constructed that simultaneously determines fluorescence intensity and excited-state lifetime. The ratio of intensity to lifetime is used to correct for the reduction in fluorescence intensity caused by collisional quenching. A frequency-domain technique is used to determine the excited-state lifetime in real-time. Computer-driven data acquisition provides instantaneous readout of the fluorescence intensity, the excited-state lifetime, and the ratio of these quantities. Correction for quenching by oxygen is demonstrated.

引用

页码：925 / 929

页数：5

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