ULTRAFAST PROTEIN DETERMINATIONS USING MICROWAVE ENHANCEMENT

被引：17

作者：

AKINS, RE

TUAN, RS

机构：

[1] ALFRED I DUPONT INST,NEMOURS RES PROGRAMS,DEPT MED CELL BIOL,WILMINGTON,DE 19899

[2] THOMAS JEFFERSON UNIV,DEPT ORTHOPED SURG,PHILADELPHIA,PA

来源：

MOLECULAR BIOTECHNOLOGY | 1995年 / 4卷 / 01期

关键词：

BIURET REACTION; LOWRY ASSAY; FOLIN-CIOCALTEAU PHENOL REAGENT; BICINCHONINIC ACID; BCA; MICROWAVE; PROTEIN ASSAY;

D O I：

10.1007/BF02907468

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present here microwave-based modifications of standard protein assays that dramatically reduce the time required to determine protein concentrations. Typical protein determinations involve incubation times ranging from 15-60 min. Microwave irradiation of specimens reduces this time requirement to 10-20 s without compromising accuracy or reliability. The remarkable speed with which protein determinations may be carried out using microwave enhancement greatly simplifies general laboratory procedures that depend on the estimation of protein concentrations.

引用

页码：17 / 24

页数：8

共 9 条

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