ROLE OF PROTEIN-KINASE-C IN THE REGULATION OF CYTOSOLIC CA-2+ IN A431 CELLS - SEPARATION OF GROWTH-FACTOR AND BRADYKININ PATHWAYS

Calcium signaling systems in nonexcitable cells involve activation of Ca2+ entry across the plasma membrane and release from intracellular stores as well as activation of Ca2+ pumps and inhibition of passive Ca2+ pathways to ensure exact regulation of free cytosolic Ca2+ concentration ([Ca2+]i). A431 cells loaded with fura-2 cells were used as a model system to examine regulation of Ca2+ entry and intracellular release. Epidermal growth factor (EGF) and transforming growth factor alpha (TGF-α) both stimulated Ca2+ entry and release while bradykinin appeared only to release Ca2+ from intracellular stores. The possible role of protein kinase C (PKC) in modulating the [Ca2+]i response to these agonists was examined by four methods. Low concentrations of TPA (2×10-10m) had no effect on Ca2+ release due to EGF, TGR-α or bradykinin but resulted in a rapid return of [Ca2+]i to baseline levels for EGF or TGF-α. Addition of the PKC inhibitor staurosporine (1 and 10 nm)_completely inhibited the action of TPA on EGF-induced [Ca2+]i changes. An inhibitor of diglyceride kinase (R59022) mimicked the action of TPA. Down-regulation of PKC by overnight incubation with 0.1 or 1 μm TPA produced the converse effect, namely prolonged Ca2+ entry following stimulation with EGF or TGF-α. To show that one effect of TPA was on Ca2+ entry, fura-2 loaded cells were suspended in Mn2+ rather than Ca2+ buffers. Addition of EGF or TGF-α resulted in Ca2+ release and Mn2+ entry. TPA but not the inactive phorbol ester, 4-α-phorbol-12,13-didecanoate, inhibited the Mn2+ influx. Thus, PKC is able to regulate Ca2+ entry due to EGF or TGF-α in this cell type. A431 cells treated with higher concentrations of TPA (5×10-8m) inhibited not only Ca2+ entry but also Ca2+ release due to EGF/TGF-α but had no effect on bradykinin-mediated Ca2+ release, suggesting differences in the regulation of the intracellular stores responsive to these two classes of agonists. Furthermore, sequential addition of EGF or TGF-α gave a single transient of [Ca2+]i, showing a common pool of Ca2+ for these agonists. In contrast, sequential addition of EGF (or TGF-α) and bradykinin resulted in two [Ca2+]i transients equal in size to those obtained with a single agonist. Ionomycin alone was able to fully deplete intracellular Ca2+ stores, whereas ionomycin following either EGF (or TGF-α) or bradykinin gave an elevation of the [Ca2+]i signal equal to that of the second agonist. These data indicate that there are separate pools of intracellular Ca2+ for EGF-mediated Ca2+ release which also respond differently to TPA. © 1990 Springer-Verlag New York Inc.