Analysis of tooth development in mice bearing a TGF-beta 1 null mutation

被引：16

作者：

DSouza, RN ^{[1
]}

Litz, M ^{[1
]}

机构：

[1] UNIV TEXAS,HLTH SCI CTR,DENT BRANCH,DEPT BASIC SCI,HOUSTON,TX 77030

来源：

CONNECTIVE TISSUE RESEARCH | 1995年 / 32卷 / 1-4期

关键词：

odontogenesis; growth factors; transforming growth factors beta; gene knockout; transgenic;

D O I：

10.3109/03008209509013704

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

While transforming growth factor-beta 1 (TGF-beta 1) and its related mammalian isoforms TGF-beta 2 and TGF-beta 3 are coexpressed in developing tooth organs, the specific biological role of each isoform is unknown. To delineate the role of TGF-beta 1 in odontogenesis, we have studied tissues from mice that lack a functional TGF-beta 1 gene. Histologic analyses revealed that in TGF-beta 1 (-/-) mice, tooth morphogenesis, cytodifferentiation and histogenesis were unaffected. Using in situ hybridization we studied the patterns of distribution of TGFs-beta 1, beta 2 and beta 3 in the TGF-beta 1 (+/+, +/- and -/-) genotypes. Our results indicate no detectable TGF-beta 1 mRNA in null tissues while TGFs-beta 2 and beta 3 showed normal temporal-spatial patterns of distribution. Using antibodies against TGF-beta 1, we observed immunoreactive TGF-beta 1 in tissues from null mice suggesting that maternally-derived TGF-beta 1 may be involved in the rescue of several developmental events in TGF-beta 1 knockout mice.

引用

页码：41 / 46

页数：6

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