CULTURE AND CRYOPRESERVATION OF CHONDROCYTES FROM HUMAN CARTILAGE - RELEVANCE FOR CARTILAGE ALLOGRAFTING IN OTOLARYNGOLOGY

被引：29

作者：

BUJIA, J

PITZKE, P

WILMES, E

HAMMER, C

机构：

[1] Department of Otolaryngology, Ludwig-Maximilians-Universität München

[2] Institute for Surgical Research, Ludwig-Maximilians-Universität München

来源：

ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY AND ITS RELATED SPECIALTIES | 1992年 / 54卷 / 02期

关键词：

RECONSTRUCTIVE SURGERY; CARTILAGE TRANSPLANTATION; CELL-TISSUE CULTURE; CRYOPRESERVATION;

D O I：

10.1159/000276267

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 [耳鼻咽喉科学];

摘要：

One of the reasons for failure of cartilage allografts is the impaired condition of the transplant during storage. In this paper we describe methods for the isolation and culture of viable chondrocytes obtained from nasal septum cartilage. Furthermore, we evaluate the possibility of growing such specific chondrocytes under culture conditions and storing them in a frozen state. Age-dependent differences were observed in the growth rate of the cultured cells. Our results confirm that chondrocytes survive freezing and remain able to proliferate. Knowledge gained from this study may be applied to the culture and freezing of viable intact cartilage for use in reconstructive surgery in otolaryngology.

引用

页码：80 / 84

页数：5

共 15 条

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