FELINE ARYLSULFATASE-B (ARSB) - ISOLATION AND EXPRESSION OF THE CDNA, COMPARISON WITH HUMAN ARSB, AND GENE LOCALIZATION TO FELINE CHROMOSOME-A1

Arylsulfatase B (ARSB) is the lysosomal enzyme that catalyzes the hydrolysis of 4-sulfate groups from N-acetylgalactosamine 4-sulfate moieties on the glycosaminoglycans, dermatan sulfate and chondroitin sulfate A. In man, a deficiency of this enzymatic activity causes the lysosomal storage disorder, Maroteaux-Lamy disease (mucopolysaccharidosis Type VI; MPS VI). MPS VI in Siamese cats also has been described, and the comparative pathologic and biochemical abnormalities of the human and feline disorders have been well characterized. The present study describes the isolation and expression of cDNAs encoding feline ARSB and the assignment of the feline ARSB gene to feline chromosome A1. The full-length feline ARSB cDNA sequence is 1939 bp, including 3 and 328 bp of 5′ and 3′ untranslated sequences, respectively, and a 1608-bp open reading frame encoding 535 amino acids. The predicted human and feline ARSB proteins are 91% identical and 94% similar. However, despite this high homology, the predicted feline ARSB polypeptide has nine cysteine residues, while the human enzyme has eight. The presence of the extra cysteine residue at position 451 in the feline enzyme may explain why feline ARSB is a homodimer and the human enzyme is a monomer. To facilitate comparative structure/function studies of the human and feline enzymes and to initiate somatic gene therapy trials in the MPS VI cats, a full-length feline ARSB cDNA was reconstructed from a 1440-bp partial cDNA and an ARSB fragment amplified from feline first-strand cDNA by the polymerase chain reaction. The functional integrity of this cDNA was demonstrated by transient expression in human embryonic kidney cells. The polymerase chain reaction also has been used to analyze 38 feline/rodent somatic cell hybrids for the presence of the feline ARSB gene. Overall, there was 97% concordancy between the presence of the feline ARSB gene and feline chromosome A1. For each of the other chromosomes, at least 34% of the hybrids were discordant. ARSB is the fifth feline gene to be assigned to chromosome A1, providing further evidence that this feline chromosome is syntenic with human chromosome 5. © 1992 Academic Press, Inc. All rights reserved.