SINGLE-TUBE REVERSE TRANSCRIPTION AND HEMINESTED POLYMERASE CHAIN-REACTION OF HEPATITIS-C VIRUS-RNA TO DETECT VIREMIA IN SEROLOGICALLY NEGATIVE HEMODIALYSIS-PATIENTS

被引：16

作者：

SAMPIETRO, M ^{[1
]}

SALVADORI, S ^{[1
]}

CORBETTA, N ^{[1
]}

BADALAMENTI, S ^{[1
]}

GRAZIANI, G ^{[1
]}

机构：

[1] OSPED MAGGIORE POLICLIN,IRCCS,DIV NEFROL & DIALISI,MILAN,ITALY

来源：

INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH | 1995年 / 25卷 / 01期

关键词：

POLYMERASE CHAIN REACTION; HEPATITIS C VIRUS; HEMODIALYSIS;

D O I：

10.1007/BF02592578

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Detection of hepatitis C virus genome is the only method currently available for detecting viremia in infected individuals. We describe a substantial simplification of current methods for viral RNA reverse transcription and polymerase chain reaction amplification, which allows cDNA synthesis and nested amplification in a single tube; this is achieved through compartmentalization of the reagents for the two reactions with a wax barrier. This procedure retains a high sensitivity: analysis of serum samples from hemodialysis patients, a high-risk group for hepatitis C virus infection, confirmed the presence of the virus in 88% of antibody-positive patients and detected viremia in patients without serological or biochemical evidence of hepatitis C virus infection.

引用

页码：52 / 54

页数：3

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