BEIJERINCKIA-INDICA VAR PENICILLANICUM PENICILLIN-V ACYLASE - ENHANCED ENZYME-PRODUCTION BY CATABOLITE REPRESSION-RESISTANT MUTANT AND EFFECT OF SOLVENTS ON ENZYME-ACTIVITY

被引：7

作者：

AMBEDKAR, SS ^{[1
]}

DESHPANDE, BS ^{[1
]}

SUDHAKARAN, VK ^{[1
]}

SHEWALE, JG ^{[1
]}

机构：

[1] HINDUSTAN ANTIBIOT LTD,RES & DEV,POONA 411018,INDIA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1991年 / 7卷 / 03期

关键词：

PENICILLIN V ACYLASE; BEIJERINCKIA-INDICA VAR PENICILLANICUM; MUTATION; SOLVENT EFFECT;

D O I：

10.1007/BF01575885

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Beijerinckia indica var. penicillanicum mutant UREMS-5, producing 168% more penicillin V acylase, was obtained by successive treatment with UV, gamma-irradiation and ethylmethane sulfonate. Penicillin V acylase production by the mutant strain was resistant to catabolite repression by glucose. Incorporation of glucose, sodium glutamate and vegetable oils in the medium enhanced enzyme production. The maximum specific production of penicillin V acylase was 244 IU/g dry weight of cells. Effect of solvents on hydrolysis of penicillin V by soluble penicillin V acylase and whole cells was studied. Methylene chloride, chloroform and carbon tetrachloride significantly stimulated the rate of penicillin V hydrolysis by whole cells.

引用

页码：209 / 214

页数：6

共 21 条

[1] BACILLUS SPHAERICUS V-PENICILLIN ACYLASE .1. FERMENTATION [J].