CHROMOSOMAL VARIATION IN DIVIDING PROTOPLASTS DERIVED FROM CELL-SUSPENSIONS OF BARLEY (HORDEUM-VULGARE L)

被引：27

作者：

WANG, XH ^{[1
]}

LAZZERI, PA ^{[1
]}

LORZ, H ^{[1
]}

机构：

[1] UNIV HAMBURG,INST GEN BOT,AMP II,OHNHORSTSTR 18,W-2000 HAMBURG 52,GERMANY

来源：

THEORETICAL AND APPLIED GENETICS | 1992年 / 85卷 / 2-3期

关键词：

CHROMOSOME VARIATION; EMBRYOGENIC REGENERABLE SUSPENSION; HORDEUM-VULGARE L; PROTOPLASTS;

D O I：

10.1007/BF00222857

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Numerical and structural chromosome variation was analysed in dividing protoplasts isolated from suspension cells of barley. Five cell lines exhibited distribution patterns in chromosome number with different peaks and ranges. Embryogenic/morphogenic cell lines showed a peak at 2n = 14 (ca. 50%) after 6 - 7 months in culture, while older non-embryogenic cell lines had peaks at aneuploid or polyploid chromosome numbers. Culture duration had a clear effect on numerical and structural chromosome variation in embryogenic cell lines. With ageing of the cultures chromosome variation accumulated and the proportion of 2n = 14 cells decreased. The effect of protoplast isolation and culture on chromosome variation was examined; more cells with normal chromosome sets (12%) were maintained in protoplast-derived colonies than in source suspension cells (4%) of the same culture age,

引用

页码：181 / 185

页数：5

共 18 条

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