EFFECT OF HIGH-FAT DIET ON THE GENE-EXPRESSION OF PANCREATIC GLUT2 AND GLUCOKINASE IN RATS

被引：53

作者：

KIM, YB ^{[1
]}

IWASHITA, S ^{[1
]}

TAMURA, T ^{[1
]}

TOKUYAMA, K ^{[1
]}

SUZUKI, M ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM, D-82152 MARTINSRIED, GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 208卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1446

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To define the biochemical and molecular basis of high-fat diet to reduce insulin secretion, the present study examined the effect of high-fat diet on the gene expression of pancreatic GLUT2, and glucokinase. Rats were isoenergetically meal-fed a high-fat or a high-carbohydrate diet for 10 weeks. Plasma glucose concentration was higher, whereas insulin concentration was lower in the rats fed a high-fat diet than in the rats fed a high-carbohydrate diet. The level of GLUT2 and glucokinase mRNA in pancreas was significantly decreased in the rats fed a high-fat diet compared with a high-carbohydrate diet. The rates of reduction in plasma insulin level, pancreatic gene expression of GLUT2 and glucokinase by high-fat feeding were about 50%. These results suggest that high-fat feeding impairs signal transduction mechanism in pancreatic beta-cells to reduce insulin secretion in rats. (C) 1995 Academic Press, Inc.

引用

页码：1092 / 1098

页数：7

共 28 条

[1]

Chalk P A, 1979, J Dev Physiol, V1, P61

[2] MECHANISM OF COMPENSATORY HYPERINSULINEMIA IN NORMOGLYCEMIC INSULIN-RESISTANT SPONTANEOUSLY HYPERTENSIVE RATS - AUGMENTED ENZYMATIC-ACTIVITY OF GLUCOKINASE IN BETA-CELLS [J].