DETERMINATION OF THE BEST NUTRIENT MEDIUM FOR THE PRODUCTION OF L-LACTIC ACID FROM BEET MOLASSES - A STATISTICAL APPROACH

被引：25

作者：

MONTEAGUDO, JM ^{[1
]}

RINCON, J ^{[1
]}

RODRIGUEZ, L ^{[1
]}

FUERTES, J ^{[1
]}

MOYA, A ^{[1
]}

机构：

[1] UNIV CASTILLA LA MANCHA, FAC CIENCIAS QUIM, DEPT CHEM ENGN, E-13071 CIUDAD REAL, SPAIN

来源：

ACTA BIOTECHNOLOGICA | 1993年 / 13卷 / 02期

关键词：

D O I：

10.1002/abio.370130204

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Optimization studies have been carried out for the production of L-lactic acid from the fermentation of beet molasses by Lactobacillus delbrueckii. A PLACKETT-BURMAN Design and a Central Composite Design have been used to determine the most suitable nutrient medium for obtaining a maximum cell concentration. A second-order polynomial empirical model relating both the cell and nutrient concentrations was formulated. The variables selected for the study were Yeast Extract, Peptone, Tween 80 (antifoam), MgSO4 . 7H2O, MnSO4.4H2O, FeSO4.7H2O and K2HPO4/KH2PO4. Among them, only Yeast Extract and Peptone were found to significantly affect the cell concentration. A maximum cell yield was found when the concentrations of Yeast Extract and Peptone were, respectively, 5.31 g/l and 5.08 g/l. All conclusions are restricted to the experimental range studied.

引用

页码：103 / 110

页数：8

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