MOLECULAR-DYNAMICS SIMULATION OF OLIGOSACCHARIDES CONTAINING N-ACETYL NEURAMINIC ACID

被引:23
作者
MUKHOPADHYAY, C
BUSH, CA
机构
[1] Department of Chemistry and Biochemistry, University of Maryland, Baltimore, Maryland, 21228
关键词
D O I
10.1002/bip.360340103
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alpha-N-acetyl neuraminic acid (Neu5Ac, sialic acid) is a commonly occurring carbohydrate residue in various cell surface glycolipids and glycoproteins. This residue is linked terminally or internally to Gal residues via an alpha(2 --> 3) or alpha(2 --> 6) linkage. In the cell surface receptor, sialyl-Lewis(X), a terminal alpha(2 --> 3) linkage is present. Previous studies from our laboratory have shown that in solution Lewis(X) adopts a relatively rigid structure. In order to model the Neu5Ac residue, vacuum molecular dynamics of this monosaccharide were compared with simulations that explicitly include solvent water. The dynamical average of the monosaccharide conformation obtained from the two simulations was similar. Vacuum calculations for the disaccharide Neu5Ac alpha(2 --> 3) Gal beta-O-methyl show that a number of low energy minima are accessible to this disaccharide. Molecular dynamics simulations starting from the low energy minima show conformational transitions with a time scale of 10-50 ps among several of the minima while large barriers between other minima prevent transitions on the time scale studied. Simulations of this disaccharide in the presence of solvent show fewer conformational transitions, illustrating a dampening effect of the solvent that has been observed in some other studies. Our results are most consistent with an equilibrium among multiple conformations for the Neu5Ac alpha(2 --> 3) Gal beta linkage. (C) 1994 John Wiley & Sons, Inc.
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页码:11 / 20
页数:10
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