The dynamic rheological properties of glutens and gluten fractions (gliadin and glutenin) of two U.K.-grown wheat cultivars, Hereward and Riband, having good and poor bread quality, respectively, were studied. Gluten and glutenin doughs from cv. Hereward had higher G' and lower tan delta values than those from cv. Riband al all frequencies studied. A more pronounced difference in G' and tan delta was observed between the glutenin doughs of the two wheats than between their respective gluten doughs. The theological properties, i.e. G' and tan delta values, of gliadin doughs were similar for both wheats. Varying the gliadin/glutenin ratio by adding the isolated gliadin or glutenin sub-fractions to the parent glutens showed that the G' values decreased and the tan delta values increased as the gliadin/glutenin ratio was increased for both cultivars, indicating a considerable decrease in elasticity as the gliadin/glutenin ratio increased. The decrease in G' may be attributed to a plasticising effect of gliadin and 'interference' of gliadin with glutenin-glutenin interactions. The reduction in G' was much more pronounced when the gliadin/glutenin ratio was increased between 0.15 and 1.0 than between 1 0 and above. Gluten from cv. Hereward had higher G' and lower tan delta values than cv. Riband gluten at all gliadin/glutenin ratios, indicating that cv. Hereward gluten had greater elastic character than cv. Riband gluten. Although significant effects of other non-protein hydrocolloid components cannot be discounted, these observations are consistent with the view that the viscoelasticity of the glutenin sub-fraction of gluten and differences in the ratio of gliadin to glutenin are the main factors governing inter-cultivar differences in the viscoelasticity of wheat gluten. (C) 1995 Academic Press Limited