HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TRIPTOLIDE AND TRIPDIOLIDE IN AN ETHYL-ACETATE EXTRACT OF TRIPTERYGIUM-WILFORDII HOOK-F

被引：19

作者：

CAI, JJ

TAO, XL

LIPSKY, PE

机构：

[1] Department of Internal Medicine, UT-Southwestern Medical Center, Dallas, Texas

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 20期

关键词：

D O I：

10.1080/10826079408013632

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new analytical method for the determination of triptolide and tripdiolide in ethyl acetate extracts of Triterygium wilfordii Hook F. is described. The procedure consists of preliminary enrichment by Sep-Pak alumina B cartridge chromatography followed by HPLC analysis. HPLC is performed with a stainless steel column packed with Nova-Pak C18, using acetonitrile-water ( 19 : 81 ) as a mobile phase for triptolide and acelonitrile-water ( 11 : 89 ) for tripdiolide. The effluent is monitored by ultraviolet detection at 214 nm. Quantitative analysis of triptolide is then carried out by comparison to an internal standard, and of tripdiolide by the external standard method. The amounts of triptolide and tripdiolide per 100 mo of the ethyl acetate extract were determined to be 19.88 ug and 9.58 ug respectively. The method is sufficiently sensitive and specific to assay the diterpenes found in Tripterygium wilfordii Hook F. accurately.

引用

页码：4479 / 4487

页数：9

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