MIGRATION BEHAVIOR AND OPTIMIZATION OF SELECTIVITY OF DICHLOROPHENOLS IN CAPILLARY ZONE ELECTROPHORESIS

The migration behaviour and selectivity of six isomeric dichlorophenols were systematically investigated using capillary zone electrophoresis. The pH, concentration of background electrolyte and applied voltage were optimized to give the best separation of these isomeric compounds. The migration behaviour and selectivity of each compound were affected strongly by pH, but much less by the composition and concentration of the electrolyte. The dependence on pH of differences in electrophoretic mobilities between two consecutively migrating solutes was used to determine the optimum buffer pH for the separation. The results confirmed that this optimum pH agrees satisfactorily with that predicted from the mean value of the solute pK(a). The pK(a) values of these six dichlorophenols area reported.