The Effect of Busulfan on Body Weight, Testis Weight and MDA Enzymes in Male Rats

Objectives: Busulfan is one of the anti-cancer drugs, despite of the tremendous usefulness in biotechnology and therapy of chronic diseases such as leukemia, lymphoma, ovarian cancer and it can lead to impaired spermatogenesis. The aim of this study was to seen body and testicular weight and serum Malondialdehyde (MDA) enzyme in rat was performed following the use of busulfan. Material and Methods: This study was conducted on 20 adult male rats ranging in age from 6 to 8 weeks, Animals in the two group includes a control group and the experimental group that received 50 mg/kg busulfan as a single intra peritoneal injection and after 8 weeks, body and testis weight and serum MDA levels were measured. Then Data were analyzed with SPSS 16. P <0.05 was considered significant, and results were compared between the two groups. Results: single dose of busulfan, induced its effects on body, testis weights and serum MDA levels. Body weight in experimental and control groups, was respectively, 297.40 gr and 301.00 gr, Body weight of rats in the experimental group than the control group was decreased, but the difference was not significant (P>0.05). Testicular weight in both control and experimental groups, respectively, were 1.45 gr and gr 0.960. Difference between the two groups was significant decreased (P>0.05). Comparison of serum MDA of control and experimental groups, were showed 3.81 +/- 1.5 and 6.9 +/- 1.1 nmol/lit. No difference between the two groups was significant increased (P>0.05). Conclusion: It can be concluded that the use of busulfan can reduced body weight and testicular weight, and increased serum MDA and could be side effects in reproduction process.