ATOMIC FORCE MICROSCOPY OF 3-DIMENSIONAL MEMBRANE-PROTEIN CRYSTALS - CA-ATPASE OF SARCOPLASMIC-RETICULUM

被引:24
作者
LACAPERE, JJ
STOKES, DL
CHATENAY, D
机构
[1] CENS,DEPT BIOL CELLULAIRE & MOLEC,CNRS,URA 1290,F-91190 GIF SUR YVETTE,FRANCE
[2] UNIV VIRGINIA,SCH MED,DEPT PHYSIOL,CHARLOTTESVILLE,VA 22908
[3] INST CURIE,PHYS & CHIM SECT,CNRS,URA 1379,F-75006 PARIS,FRANCE
[4] UNIV PARIS 06,F-75006 PARIS,FRANCE
关键词
D O I
10.1016/S0006-3495(92)81600-4
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
We have observed three-dimensional crystals of the calcium pump from sarcoplasmic reticulum by atomic force microscopy (AFM). From AFM images of dried crystals, both on graphite and mica, we measured steps in the crystal thickness, corresponding to the unit cell spacing normal to the substrate. It is known from transmission electron microscopy that crystal periodicity in the plane of the substrate is destroyed by drying, and it was therefore not surprising that we were unable to observe this periodicity by AFM. Thus, we were motivated to use the AFM on hydrated crystals. In this case, crystal adsorption appeared to be a limiting factor, and our studies indicate that adsorption is controlled by the composition of the medium and by the physical-chemical properties of the substrate. We used scanning electron microscopy to determine the conditions yielding the highest adsorption of crystals, and, under these conditions, we have obtained AFM images of hydrated crystals with a resolution similar to that observed with dried samples (i.e., relatively poor). In the same preparations, we have observed lipid bilayers with a significantly better resolution, indicating that the poor quality of crystal images was not due to instrumental limitations. Rather, we attribute poor images to the intrinsic flexibility of these multilamellar crystals, which apparently allow movement of one layer relative to another in response to shear forces from the AFM tip. We therefore suggest some general guidelines for future studies of membrane proteins with AFM.
引用
收藏
页码:303 / 308
页数:6
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