DETERMINATION OF GLYCEROL IN BACTERIAL-CELL WALL TEICHOIC-ACID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：2

作者：

HIMANEN, JP ^{[1
]}

机构：

[1] NATL PUBL HLTH INST,MOLEC BIOL PROGRAMME,SF-00300 HELSINKI,FINLAND

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 607卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)87047-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatography method for the determination of glycerol in teichoic acid was developed. The phosphodiester linkages between repeating glycerol units were hydrolysed by treatment with 5.8 M HCl at 100-degrees-C for 75 h. The degree of hydrolysis was verified by gas chromatography-mass spectrometry. The hydrolysed samples were applied to an ion-exclusion column and the amount of glycerol determined as the area of the peak at 12.6 min. Using glycerol standards an r2 value of 0.999, a relative standard deviation of 2.2% and a detection limit of 0.1-mu-g were obtained.

引用

页码：1 / 6

页数：6

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