ROLE OF NITRIC-OXIDE IN MODULATING PERMEABILITY OF HAMSTER-CHEEK POUCH IN RESPONSE TO ADENOSINE 5'-DIPHOSPHATE AND BRADYKININ

被引:120
作者
MAYHAN, WG
机构
[1] Department of Physiology and Biophysics, University of Nebraska Medical Center, Omaha, 68198-4575, Nebraska
关键词
D O I
10.1007/BF00917622
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The goal of this study was to determine the role of the synthesis and release of nitric oxide in modulating alterations in microvascular permeability of the hamster cheek pouch in response to adenosine 5'-diphosphate and bradykinin. We used intravital fluorescent microscopy to examine the permeability of the hamster cheek pouch to agonists before and following application of enzymatic inhibitors of nitric oxide, N(G)-monomethyl-L-arginine (L-NMMA; 0.01, 0. 1, and 1.0-mu-M) and N(w)-nitro-L-arginine methyl ester (L-NAME; 0.01, 0. 1, and 1.0-mu-M). Increases in permeability of the hamster cheek pouch were quantitated by the formation of microvascular leaky sites. ADP and bradykinin produced an increase in the number of venular leaky sites, and superfusion of L-NMMA and L-NAME significantly decreased ADP- and bradykinin-induced increases in microvascular permeability. To determine the specificity of nitric oxide blockade on microvascular permeability, we examined changes in permeability in response to adenosine, and examined the effects of D-NMMA on microvascular permeability. Adenosine-induced increases in permeability were not altered by treatment with L-NMMA, and D-NMMA did not inhibit ADP-induced increases in microvascular permeability. Thus, these findings suggest that production of nitric oxide, in response to application of ADP and bradykinin, has a role in modulating macromolecular permeability of the hamster cheek pouch in vivo.
引用
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页码:295 / 305
页数:11
相关论文
共 27 条
[1]   PHYSIOLOGICAL IMPORTANCE OF NITRIC-OXIDE [J].
COLLIER, J ;
VALLANCE, P .
BRITISH MEDICAL JOURNAL, 1991, 302 (6788) :1289-1290
[2]   ENDOTHELIUM-DERIVED RELAXING AND CONTRACTING FACTORS [J].
FURCHGOTT, RF ;
VANHOUTTE, PM .
FASEB JOURNAL, 1989, 3 (09) :2007-2018
[3]   DOSE-RELATED EFFECTS OF ADENOSINE AND BRADYKININ ON MICROVASCULAR PERMSELECTIVITY TO MACROMOLECULES IN THE HAMSTER-CHEEK POUCH [J].
GAWLOWSKI, DM ;
DURAN, WN .
CIRCULATION RESEARCH, 1986, 58 (03) :348-355
[4]   ADENINE-NUCLEOTIDES, SEROTONIN, AND ENDOTHELIUM-DEPENDENT RELAXATIONS TO PLATELETS [J].
HOUSTON, DS ;
SHEPHERD, JT ;
VANHOUTTE, PM .
AMERICAN JOURNAL OF PHYSIOLOGY, 1985, 248 (03) :H389-H395
[5]   EVIDENCE THAT ENDOGENOUS NITRIC-OXIDE MODULATES EDEMA FORMATION INDUCED BY SUBSTANCE-P [J].
HUGHES, SR ;
WILLIAMS, TJ ;
BRAIN, SD .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1990, 191 (03) :481-484
[6]   INTRAVITAL AND ELECTRON-MICROSCOPIC STUDY OF BRADYKININ-INDUCED VASCULAR-PERMEABILITY CHANGES USING FITC-DEXTRAN AS A TRACER [J].
HULSTROM, D ;
SVENSJO, E .
JOURNAL OF PATHOLOGY, 1979, 129 (03) :125-&
[7]   BIOSYNTHESIS AND METABOLISM OF ENDOTHELIUM-DERIVED NITRIC-OXIDE [J].
IGNARRO, LJ .
ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1990, 30 :535-560
[8]   ENDOTHELIAL CONTRACTION INDUCED BY HISTAMINE-TYPE MEDIATORS - AN ELECTRON MICROSCOPIC STUDY [J].
MAJNO, G ;
SHEA, SM ;
LEVENTHA.M .
JOURNAL OF CELL BIOLOGY, 1969, 42 (03) :647-&
[9]   STUDIES ON INFLAMMATION .1. EFFECT OF HISTAMINE AND SEROTONIN ON VASCULAR PERMEABILITY - AN ELECTRON MICROSCOPIC STUDY [J].
MAJNO, G ;
PALADE, GE .
JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY, 1961, 11 (03) :571-&
[10]   RAT MAST-CELLS SYNTHESIZE A NITRIC-OXIDE LIKE-FACTOR WHICH MODULATES THE RELEASE OF HISTAMINE [J].
MASINI, E ;
SALVEMINI, D ;
PISTELLI, A ;
MANNAIONI, PF ;
VANE, JR .
AGENTS AND ACTIONS, 1991, 33 (1-2) :61-63