THE US3 PROTEIN-KINASE OF HERPES-SIMPLEX VIRUS TYPE-2 IS ASSOCIATED WITH PHOSPHORYLATION OF THE UL12 ALKALINE NUCLEASE IN-VITRO

被引：23

作者：

DAIKOKU, T

YAMASHITA, Y

TSURUMI, T

NISHIYAMA, Y

机构：

[1] Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya

来源：

ARCHIVES OF VIROLOGY | 1995年 / 140卷 / 09期

关键词：

D O I：

10.1007/BF01322537

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Herpes simplex virus type 2 (HSV-2) gene US3 encodes a serine-threonine protein kinase. We previously described the isolation of a US3-inactivated mutant which is able to replicate in Vero cells but not in murine macrophages. To learn more about the biological role of the US3 protein kinase, we have sought to identify the target proteins of the enzyme. Studies of in vitro phosphorylation with extracts of infected cells demonstrate that the US3 protein kinase is involved in phosphorylation of the UL12 alkaline nuclease in vitro, suggesting that the nuclease is a possible target of the protein kinase.

引用

页码：1637 / 1644

页数：8

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