A NEW PROCESS FOR IMPROVING THE QUALITY AND LIGNIN STAINING OF ULTRATHIN SECTIONS FROM WOOD TISSUE

被引：26

作者：

MAURER, A

FENGEL, D

机构：

[1] Institut für Holzforschung, Universität München, D-8000 München 40, Federal

来源：

HOLZFORSCHUNG | 1990年 / 44卷 / 06期

关键词：

TRANSMISSION ELECTRON MICROSCOPY; EMBEDDING; MELAMINE RESIN; ULTRATHIN SECTIONS; LIGNIN STAINING; POTASSIUM PERMANGANATE; LIGNIN DISTRIBUTION; DENSITOMETRY; PICEA-ABIES (L) KARST; FAGUS-SYLVATICA L;

D O I：

10.1515/hfsg.1990.44.6.453

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

In this study a new combination of embedding and staining methods was developed which permits the visualization of the lignin distribution in the cell walls at the high resolution level of a TEM. The use of water soluble melamine resin for embedding results in extremely ultrathin sections which can be observed in the TEM without supporting films. The embedding allows a staining, specifically of lignin, with KMnO4 with high reproducibility. A densitometric evaluation of the micrographs reveals a great number of details about the lignin concentration within the various cell wall layers.

引用

页码：453 / 460

页数：8

共 33 条

[1] ELECTRON-MICROSCOPY OF MELAMINE-EMBEDDED FROG RETINA - EVIDENCE FOR THE OVERALL CRYSTALLINE ORGANIZATION OF PHOTORECEPTOR OUTER SEGMENTS [J].