NEW CYCLODEPSIPEPTIDES, ENNIATIN-D, ENNIATIN-E AND ENNIATIN-F PRODUCED BY FUSARIUM SP FO-1305

New cyclodepsipeptides named enniatins D, E and F were isolated from the culture broth of Fusarium sp. FO-1305 as inhibitors of acyl-CoA: cholesterol acyltransferase (ACAT). The respective structures of enniatins D, E and F were determined to be cyclo[D-alpha-hydroxyisovaleryl(D-Hiv)-L-N-methylleucinyl(L-Me-Leu)-D-Hiv-L-N-methylvalinyl(L-Me-Val)-D-HiV-L-Me-Val], a mixture of cyclo-[D-HiV-L-Me-Leu-D-Hiv-L-N-methylisoleucinyl(L-Me-Ile)-D-Hiv-L-Me-Val] and cyclo(D-Hiv-L-Me-Ile-D-HiV-L-Me-Leu-D-HiV-L-Me-Val), and cyclo(D-HiV-L-MC-LeU-D-Hiv-L-Me-Ile-D-HiV-L-Me-Ile) by spectral analyses and chemical degradation. The IC50 values of enniatins D, E and F for ACAT activity in an enzyme assay using rat liver microsomes were calculated to be 87, 57 and 40-mu-M, respectively.