HEPATITIS-DELTA VIRUS-RNA IN SERUM OF PATIENTS WITH CHRONIC DELTA-HEPATITIS

被引：4

作者：

SIMPSON, LH ^{[1
]}

BATTEGAY, M ^{[1
]}

HOOFNAGLE, JH ^{[1
]}

WAGGONER, JG ^{[1
]}

DIBISCEGLIE, AM ^{[1
]}

机构：

[1] NIDDK,LIVER DIS SECT,BETHESDA,MD 20892

来源：

DIGESTIVE DISEASES AND SCIENCES | 1994年 / 39卷 / 12期

关键词：

HEPATITIS DELTA VIRUS; HOT START TECHNIQUE; POLYMERASE CHAIN REACTION; CHRONIC HEPATITIS; VIREMIA;

D O I：

10.1007/BF02087704

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

We studied 28 patients with chronic delta hepatitis for the presence of hepatitis delta virus (HDV) RNA in serum. The hot start polymerase chain reaction (PCR) method, in which the reaction begins at 60-80 degrees C, showed a higher sensitivity than conventional PCR reaction. Additionally, the presence of hepatitis B (HBV) and C virus (HCV) infections were determined by PCR. HDV RNA was detected in 26 patients (93%), HBV DNA in 22 (79%), and HCV RNA in only one. Detection of HDV RNA correlated very well with detection of hepatitis delta antigen by immunostaining in the liver. In six patients HDV RNA was detectable despite the absence of HBV DNA in serum, suggesting that high levels of HBV are not required for HDV replication. Of 29 control patients with chronic hepatitis B without antibody to HDV, none had detectable HDV RNA, while all had HBV DNA in serum. Detection of HDV RNA with PCR proved highly sensitive and specific, demonstrating that virtually all patients with chronic HDV infection had ongoing viral replication.

引用

页码：2650 / 2655

页数：6

共 30 条

[1] CHRONIC DELTA HEPATITIS - DETECTION OF HEPATITIS DELTA-VIRUS ANTIGEN IN SERUM BY IMMUNOBLOT AND CORRELATION WITH OTHER MARKERS OF DELTA-VIRAL REPLICATION [J].