CLONING OF THE HUMAN BRAIN GABA TRANSPORTER

被引：157

作者：

NELSON, H

MANDIYAN, S

NELSON, N

机构：

[1] Roche Institute of Molecular Biology, Roche Research Center, Nutley

来源：

FEBS LETTERS | 1990年 / 269卷 / 01期

关键词：

cDNA; sequence; Human; Neurotransmitter; Transport; γ-Aminobutric acid;

D O I：

10.1016/0014-5793(90)81149-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone encoding a transporter for the neurotransmitter γ-aminobutyric acid in human brain was cloned and sequenced. The cDNA contains an open reading frame encoding a hydrophobic protein of 599 amino acids with a calculated molecular weight of 67022 Da. Hydropathy analysis revealed twelve potential transmembrane segments. The human protein is highly homologous to the protein from rat brain. Northern hybridization demonstrated a ubiquitous distribution of the transporter in various parts of the brain. © 1990.

引用

页码：181 / 184

页数：4

共 14 条

[1]

GUASTELLA J, 1990, IN PRESS SCIENCE

[2] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].