IDENTIFICATION OF POLYPEPTIDE MARKERS OF BARLEY YELLOW DWARF VIRUS-RESISTANCE AND SUSCEPTIBILITY GENES IN NONINFECTED BARLEY (HORDEUM-VULGARE) PLANTS

被引:8
作者
HOLLOWAY, PJ
HEATH, R
机构
[1] Burnley Gardens, Institute of Plant Sciences, Burnley, 3121, Victoria, Swan Street
关键词
BARLEY YELLOW DWARF VIRUS; RESISTANCE GENE(S); HIGH-RESOLUTION; 2-DIMENSIONAL ELECTROPHORESIS; BARLEY; WESTERN IMMUNOBLOT;
D O I
10.1007/BF00222880
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Barley yellow dwarf (BYDV) is a group a closely related viruses which cause economic losses in a wide range of graminaceous species throughout the world. Barley plants can be protected from the effects of BYDV by the 'Yd2' resistance gene. Plants which contain the 'Yd2' gene also contain a constitutively expressed polypeptide which was not found in any plants without 'Yd2'. Conversely, BYDV susceptible plants contain another constitutively expressed polypeptide which was not found in any of the BYDV-resistant lines examined. These two polypeptides appear to have the same molecular weight (as assessed by SDS-PAGE) and only slightly different iso-electric points. They also appear to contain an extensive range of similar antigenic determinants. Both polypeptides were found in F1 hybrids made from resistant and susceptible plants. We suggest that these two polypeptides are the products of two allelic genes. Analysis of near-isogenic lines showed that the locus which controls the 'Yd2' resistance gene and the locus controlling the synthesis of the two polypeptides may be within +/- 9 cM of each other. We have developed a Western blot technique which allows assessment of barley lines, 4-days after seed imbibition, for the presence of the 'Yd2' gene.
引用
收藏
页码:346 / 352
页数:7
相关论文
共 28 条
[1]   OPTIMAL CONDITIONS FOR THE USE OF CDNA PROBES TO MEASURE THE CONCENTRATION OF BARLEY YELLOW DWARF VIRUS IN BARLEY (HORDEUM-VULGARE) [J].
ARUNDEL, PH ;
VANDEVELDE, R ;
HOLLOWAY, PJ .
JOURNAL OF VIROLOGICAL METHODS, 1988, 19 (3-4) :307-318
[2]  
BURNET PA, 1987, 1987 P INT WORKSH JU, pR9
[3]   Inheritance and effectiveness of genes in barley that condition tolerance to barley yellow dwarf virus [J].
Catherall, P. L. ;
Jones, A. T. ;
Hayes, J. D. .
ANNALS OF APPLIED BIOLOGY, 1970, 65 (01) :153-161
[4]  
Chua N. H., 1980, METHOD ENZYMOL, V69, P434, DOI 10.1016/S0076-6879(80)69042-9
[5]  
CONTI M, 1987, 1990 WORLD PERSP BAR, P1
[6]  
DAMSTEEGT VD, 1964, PHYTOPATHOLOGY, V54, P219
[7]   IDENTIFICATION AND MOLECULAR MAPPING OF A SINGLE ARABIDOPSIS-THALIANA LOCUS DETERMINING RESISTANCE TO A PHYTOPATHOGENIC PSEUDOMONAS-SYRINGAE ISOLATE [J].
DEBENER, T ;
LEHNACKERS, H ;
ARNOLD, M ;
DANGL, JL .
PLANT JOURNAL, 1991, 1 (03) :289-302
[8]  
Eagling D. R., 1991, Review of Plant Pathology, V70, P133
[9]   ACID-PHOSPHATASE TYPING FOR BREEDING NEMATODE-RESISTANT TOMATOES BY ISOELECTRIC-FOCUSING WITH AN ULTRANARROW IMMOBILIZED PH GRADIENT [J].
GUNTHER, S ;
POSTEL, W ;
WIERING, H ;
GORG, A .
ELECTROPHORESIS, 1988, 9 (09) :618-620
[10]   HIGH-RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS OF PLANT-PROTEINS [J].
HOLLOWAY, PJ ;
ARUNDEL, PH .
ANALYTICAL BIOCHEMISTRY, 1988, 172 (01) :8-15