CLONING AND SEQUENCING OF THE BETA-LACTAM HYDROXYLASE GENE (CEFF) FROM STREPTOMYCES-CLAVULIGERUS - GENE DUPLICATION MAY HAVE LED TO SEPARATE HYDROXYLASE AND EXPANDASE ACTIVITIES IN THE ACTINOMYCETES

被引：55

作者：

KOVACEVIC, S ^{[1
]}

MILLER, JR ^{[1
]}

机构：

[1] ELI LILLY INT CORP,LILLY RES LAB,DEPT MOLEC GENET RES,LILLY CORP CTR,INDIANAPOLIS,IN 46285

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 01期

关键词：

D O I：

10.1128/jb.173.1.398-400.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an M(r) of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opposite orientation. The hydroxylase and expandase genes are 59 and 71% identical at the amino acid and DNA levels, respectively. cefE and cefF may have arisen from a gene duplication in the actinomycetes.

引用

页码：398 / 400

页数：3

共 20 条

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