T-CELL RECEPTOR V-GENE USAGE OF ISLET BETA-CELL REACTIVE T-CELLS IS NOT RESTRICTED IN NONOBESE DIABETIC MICE

被引：149

作者：

NAKANO, N

KIKUTANI, H

NISHIMOTO, H

KISHIMOTO, T

机构：

[1] OSAKA UNIV,INST MOLEC & CELLULAR BIOL,DIV IMMUNOL,1-3 YAMADA-OKA,SUITA,OSAKA 565,JAPAN

[2] SHIONOGI & CO LTD,SHIONOGI RES LAB,OSAKA 553,JAPAN

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 1991年 / 173卷 / 05期

关键词：

D O I：

10.1084/jem.173.5.1091

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Five islet-reactive T cell clones were established from islet-infiltrating T cells of non-obese diabetic (NOD) mice. All clones expressed CD4, but not CD8, and responded to islet cells from various strains of mice in the context of I-A(NOD). They could induce insulitis when transferred into disease-resistant I-E+ transgenic NOD mice. The T cell receptor (TCR) sequences utilized by the clones were determined. Their usage of TCR V and J segments was not restricted but was rather diverse. One of the clones utilized V-beta-16. The expression of V-beta-16 was significantly reduced in I-E+ transgenic NOD, suggesting the possibility that the islet-reactive T cell clone expressing V-beta-16 may be deleted or inactivated by I-E molecules. This clone might be one of the candidates that triggers insulitis.

引用

收藏

页码：1091 / 1097

页数：7

相关论文

共 30 条

[1] THE 1ST EXTERNAL DOMAIN OF THE NONOBESE DIABETIC MOUSE CLASS-II I-A BETA-CHAIN IS UNIQUE [J].

ACHAORBEA, H ;

MCDEVITT, HO .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (08) :2435-2439

[2] LIMITED HETEROGENEITY OF T-CELL RECEPTORS FROM LYMPHOCYTES MEDIATING AUTOIMMUNE ENCEPHALOMYELITIS ALLOWS SPECIFIC IMMUNE INTERVENTION [J].

ACHAORBEA, H ;

MITCHELL, DJ ;

TIMMERMANN, L ;

WRAITH, DC ;

TAUSCH, GS ;

WALDOR, MK ;

ZAMVIL, SS ;

MCDEVITT, HO ;

STEINMAN, L .

CELL, 1988, 54 (02) :263-273

[3] SYNGENEIC TRANSFER OF AUTOIMMUNE DIABETES FROM DIABETIC NOD MICE TO HEALTHY NEONATES - REQUIREMENT FOR BOTH L3T4+ AND LYT-2+ T-CELLS [J].

BENDELAC, A ;

CARNAUD, C ;

BOITARD, C ;

BACH, JF .

JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 166 (04) :823-832

[4] AN ANALYSIS OF T-CELL RECEPTOR VARIABLE REGION GENE-EXPRESSION IN MAJOR HISTOCOMPATIBILITY COMPLEX DISPARATE MICE [J].

BILL, J ;

APPEL, VB ;

PALMER, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :9184-9188

[5] THE MHC MOLECULE I-E IS NECESSARY BUT NOT SUFFICIENT FOR THE CLONAL DELETION OF V-BETA-11-BEARING T-CELLS [J].

BILL, J ;

KANAGAWA, O ;

WOODLAND, DL ;

PALMER, E .

JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 169 (04) :1405-1419

[6] MHC-LINKED PROTECTION FROM DIABETES DISSOCIATED FROM CLONAL DELETION OF T-CELLS [J].

BOHME, J ;

SCHUHBAUR, B ;

KANAGAWA, O ;

BENOIST, C ;

MATHIS, D .

SCIENCE, 1990, 249 (4966) :293-295

[7] THE PRESUMPTIVE CDR3 REGIONS OF BOTH T-CELL RECEPTOR ALPHA-CHAIN AND BETA-CHAIN DETERMINE T-CELL SPECIFICITY FOR MYOGLOBIN PEPTIDES [J].

DANSKA, JS ;

LIVINGSTONE, AM ;

PARAGAS, V ;

ISHIHARA, T ;

FATHMAN, CG .

JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (01) :27-33

[8] BETA-CELL LINES DERIVED FROM TRANSGENIC MICE EXPRESSING A HYBRID INSULIN GENE ONCOGENE [J].

EFRAT, S ;

LINDE, S ;

KOFOD, H ;

SPECTOR, D ;

DELANNOY, M ;

GRANT, S ;

HANAHAN, D ;

BAEKKESKOV, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :9037-9041

[9] CORRELATIONS BETWEEN T-CELL SPECIFICITY AND THE STRUCTURE OF THE ANTIGEN RECEPTOR [J].

FINK, PJ ;

MATIS, LA ;

MCELLIGOTT, DL ;

BOOKMAN, M ;

HEDRICK, SM .

NATURE, 1986, 321 (6067) :219-226

[10] PANCREATIC ISLET-SPECIFIC T-CELL CLONES FROM NONOBESE DIABETIC MICE [J].

HASKINS, K ;

PORTAS, M ;

BERGMAN, B ;

LAFFERTY, K ;

BRADLEY, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (20) :8000-8004